The influence of synthetic conditions on the stability of methotrexate-monoclonal antibody conjugates determined by reversed phase high performance liquid chromatography.

Methotrexate (MTX) has been convalently attached to an IgG-type monoclonal antibody (791T/36) directed to tumour-associated antigen gp72. Conjugates were synthesized by the active ester method using MTX N-succinimidyl ester at various pH values (7.5-10.5). Following purification by gel filtration, high performance liquid chromatography was used to assess the free drug or its derivatives in samples of MTX-791T/36 conjugates previously treated (or not) with hydroxylamine. Quantitative analysis, performed on a reversed phase column (pore size 300 A) with isocratic acetonitrile-sodium acetate buffer (pH 4.8) as mobile phase, indicated no detectable amount of free methotrexate in hydroxylamine-treated conjugates even six months after their preparation. Similar observations were made with conjugates, whose synthesis were performed at pH greater than or equal to 10. In contrast, the presence of increasing amounts of drug/metabolite could be demonstrated in samples produced at lower pH values. Based on these findings, the pH-dependent kinetics of MTX release has been determined and used to design conditions under which stable MTX-791T/36 conjugates could be prepared without hydroxylamine reaction.     

Studies on recoil proton tracks in NTA films with the T(d,n)He reaction

Recoil proton tracks in nuclear emulsion are counted by scanning them using strip, square or circular field of view of optical microscope. In this paper, the overestimate due to the edge effects in counting recoil proton tracks at different neutron energies produced from the T(d,n)⁴He reaction has been determined by measuring the true track density in NTA film. The overestimate has also been calculated using the measured value of the mean projected track length (L) in the film. The percentage of measured overestimates has been compared with those obtained by calculation and the results agree reasonably well. Fading effect in NTA film has been studied and found to be 22% more in the summer than in the winter season. A great reduction in fading rate could be achieved if the films are desiccated and sealed in highly pure dry nitrogen maintained at an over-pressure of 1000 Pa to reduce water vapour ingress. After the edge effect correction, the sensitivity of NTA film has been calculated from (1.72±0.08)×10⁻³ tracksn⁻¹ to (1.97±0.16)×10⁻³ tracksn⁻¹ in the neutron energy ranges from 15.91 to 18.88 MeV. The response was found to be from 0.24±0.02 trackscm⁻² permSv to 0.26±0.01 trackscm⁻² permSv in the same energy range.     

MITATT mode in DDR heterostructure Impatt

Large signal characterisation of double heterostructure DDR Impatt diode has been carried out in the millimeter-wave range considering the MITATT mode of operation. The structure of the device is p⁺-p₂-p₁-n₁-n₂-n⁺ where impact ionisation and tunneling takes place in the p₁-n₁ region. In this study we have considered two well-known heterostructures, e.g., InP/GaInAs/InP and InP/InGaAsP/InP and one nonconventional structure GaAs/InP/GaAs. The theoretical results of the performances of these devices as regards of output power, efficiency, and negative conductance revealed that the structures are quite promising as the source of power in the millimeter-wave range. The analysis may be used for other mm wave DDR heterostructure Impatts.     

An optimal repeat inspection plan with several classifications

Multicharacteristic critical components exist in many systems. Such components could be a part of an aircraft, space shuttle or a gas ignition system. A component is critical if it causes disaster or a very high cost upon failure. In this paper, a new inspection plan for critical multicharacteristic components is presented. A mathematical model that depicts the plan is developed. An algorithm is proposed for finding the optimal number of repeat inspections and the sequence of characteristics for inspection that minimizes expected total cost per accepted component. The expected cost consists of the cost of inspection and the cost of misclassifications. The inspection plan and the model developed generalize existing models in the literature and provide a more realistic formulation. An example is given to demonstrate the plan and the model.     

Poly(ortho‐phenylene ethynylene)s: Synthetic accessibility and optical properties

Poly(ortho‐phenylene ethynylene)s (PoPEs) have been synthesized via an in situ activation/coupling AB′ polycondensation protocol. The resulting polymers have been characterized by several analytical methods and are shown to have no structural defects. Although the Sonogashira–Hagihara polycondensation reaction is less efficient than for the preparation of the corresponding meta‐ and para‐linked polymers, presumably because of steric hindrance caused by the ortho substituents, the process can be accelerated by the use of microwave irradiation. Optical spectroscopy indicates solvent‐dependent conformational changes between extended transoid and helical cisoid conformations, providing the first experimental evidence for solvophobically driven folding of the PoPE backbone. © 2006 Wiley Periodicals, Inc. J Polym Sci Part A: Polym Chem 44: 1619–1627, 2006     

Differential oscillator strengths and dipole polarizabilities for transitions of the helium sequence

The dipole radial integral for an initial discrete 1s state and a final continuum state has been calculated under the screened hydrogenic model. In this model, single-electron hydrogenic wave functions are employed and the initial and the final states are treated by two different effective-charge parameters. Numerical values of differential oscillator strengths for transitions from 1s 21S to the continuum for the helium sequence ions are obtained. Also calculated are the dipole polarizabilities, which are found to be in excellent agreement with the results of other authors.     

Natural and fallout radionuclide concentrations in the environment of Islamabad

A study on the concentration of natural and fallout radionuclides in environmental samples collected from different localities of Islamabad was performed. For the determination of gamma-emitters such as ²³⁸U, ²³²Th, ⁴⁰K and ¹³⁷Cs high purity germanium (HPGe) detector was used while for the analysis of ⁹⁰Sr, a beta-emitter, liquid scintillation counting system was used. The indoor absorbed dose rate was measured by a CaF₂ : Dy thermoluminescence detector. Other radiation parameters were also determined to evaluate the radiation hazard. All the results were well within the permissible limits showing that there is no radiation hazard in the environment of Islamabad.     

Two New and a Known Compound from Lawsonia inermis

A new obtusafuran derivative, lawsonicin ( 1 ), and a new naphthaquinone, lawsonadeem ( 2 ), along with a known constituent, vomifoliol ( 3 ), were isolated from the aerial parts of Lawsonia alba and characterized by chemical transformation and spectroscopic experiments, including 2D‐NMR techniques.     

Terpenoids of the essential oil ofLedum hypoleucum

The chemical composition of the terpenoids of the essential oil ofLedum hypoleucum growing in the Far East has been studied. It differs fromL. palustre by a larger amount of compounds of the bisabolane series in the essential oil. Among the monoterpenoids, p-cymene, -phellandrene, and ascaridole predominate. The main components of the sesquiterpenoids are ar-curcumene and ar-turmerone, now found for the first time inLedum essential oils.Tomsk State Medical Institute. Novosibirsk Institute of Organic Chemistry, Siberian Branch, Academy of Sciences of the USSR. Translated from Khimiya Prirodnykh Soedinenii, No. 1, pp. 104–107, January–February, 1987.